文章摘要
郑利伟.双波长HPLC法同时测定小儿清肺止咳片中栀子苷和黄芩苷的含量[J].中国医药导刊,2019,21(6):365-368.
双波长HPLC法同时测定小儿清肺止咳片中栀子苷和黄芩苷的含量
Simultaneous Determination of Geniposide and Baicalin in Xiaoer Qingfei Zhike Tablets by Dual Wavelength HPLC
投稿时间:2019-04-22  修订日期:2019-05-15
DOI:
中文关键词: 小儿清肺止咳片  栀子苷  黄芩苷  HPLC  含量测定
英文关键词: Xiaoer qingfei zhike tablets  Geniposide  Baicalin  HPLC  Content determination
基金项目:
作者单位E-mail
郑利伟 铁岭市药品检验所 979021815@qq.com 
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中文摘要:
      目的:建立同时测定小儿清肺止咳片中栀子苷和黄芩苷含量的方法。方法:采用双波长高效液相色谱(HPLC)法,以Agilent ZORBAX C18色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-0.3%磷酸溶液进行梯度洗脱,检测波长240 nm(栀子苷),278 nm(黄芩苷),柱温30 ℃,进样量为10 μL。结果:栀子苷和黄芩苷分别在0.081~0.65 μg·mL-1(r=0.999 6)、0.10~0.81 μg·mL-1(r=0.999 7)浓度范围内有良好的线性关系。栀子苷和黄芩苷的平均回收率(n=6)分别为97.8%,98.2%,RSD分别为0.5%和0.8%。结论:本方法简便、准确、回收率好,可用于小儿清肺止咳片中栀子苷和黄芩苷的含量测定和质量控制。
英文摘要:
      Objective: To establish a method for simultaneous determination of geniposide and baicalin in xiaoer qingfei zhike tablets. Methods: Using dual wavelength HPLC method, the chromatographic column was Agilent ZORBAX C18(4.6 mm×250 mm, 5 μm), mobile phase of acetonitrile-0.3% phosphoric acid solution with gradient elution, detection wavelength was 240 nm (gardenoside) and 278 nm (baicalin), column temperature was 30 ℃, injection volume was 10 μL. Results: Geniposide and baicalin showed a good linear relationship in the concentration range of 0.081-0.65 μg·mL-1 (r=0.999 6) and 0.10-0.81 μg·mL-1 (r=0.999 7), respectively. The average recovery rate of geniposide and baicalin (n=6) were 97.8%, 98.2% respectively(RSD 0.5% and 0.8%). Conclusion: The method is simple, accurate and has good recovery rate. It is suitable for the content determination and quality control of xiaoer qingfei zhike tablets.
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