文章摘要
邬秋萍,万林春.HPLC法测定地锦草中游离和总没食子酸含量[J].中国医药导刊,2017,19(9):943-945.
HPLC法测定地锦草中游离和总没食子酸含量
Quantitative Determination of Free Gallic Acid and Total Gallic Acid in Euphorbiae Humifusae Herba by HPLC
投稿时间:2017-08-02  修订日期:2017-08-02
DOI:
中文关键词: 地锦草  高效液相色谱  游离没食子酸  总没食子酸
英文关键词: Euphorbiae Humifusae Herba  High performance liquid chromatography  Free gallic acid  Total gallic acid
基金项目:江西省科技计划项目(项目编号:20151BBG70040;项目名称:肠炎宁片中地锦草炮制工艺及其质量标准研究)
作者单位E-mail
邬秋萍 江西省药品检验检测研究院 44266174@qq.com 
万林春 江西省药品检验检测研究院 425217982@qq.com 
摘要点击次数: 105
全文下载次数: 0
中文摘要:
      目的:建立HPLC法测定地锦草中游离没食子酸和总没食子酸的含量。方法:采用Agela Odyssil C18(4.6 mm×250 mm,5 μm)色谱柱;流动相为甲醇-0.5%磷酸溶液(8∶92);检测波长270 nm;流速1.0 mL·min-1;柱温30 ℃。结果:没食子酸在0.034 0~1.700 2 μg范围内呈良好线性关系(r=1.000 0),游离没食子酸和总没食子酸平均回收率分别为99.63%和99.34%。结论:该方法操作简便准确,灵敏度高,重复性好,可用于地锦草中没食子酸的质量控制。
英文摘要:
      Objective:To establish a method for quantitative determination of free gallic acid and total gallic acid in Euphorbiae Humifusae Herba by HPLC.Methods:The HPLC procedure was performed on the chromatographic column of Agela Odyssil C18(4.6 mm×250 mm, 5μm)at 30℃, the mobile phase was methanol-0.5% phosphoric acid solution(8∶92)with the flow velocity of 1.0 mL·min-1, and the detection wavelength was 270 nm.Results: Gallic acid showed a good linear relationship with peak area at a range from 0.0340 μg to 1.7002 μg(r= 1.0000), the free gallic acid recovery was 99.63% and the total gallic acid recovery was 99.34%.Conclusion:The method is simple, accurate with high sensitivity and good repeatability, and can be used for the quality control of gallic acid in Euphorbiae Humifusae Herba.
查看全文   查看/发表评论  下载PDF阅读器